| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.093852 |
| Chromosome: | chromosome 2 |
| Location: | 950507 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079850 | POLK1 | (1 of 1) K03511 - DNA polymerase kappa (POLK); DNA polymerase kappa | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGCGGCTGCTGCTCCCCGCTCTCCGTA |
| Internal bar code: | TATTCGGTCTGGATTTCTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 118 |
| LEAP-Seq percent confirming: | 76.0568 |
| LEAP-Seq n confirming: | 2195 |
| LEAP-Seq n nonconfirming: | 691 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGTGCCCTAAGCGTAT |
| Suggested primer 2: | GATTATGGTTCAGGCCGCTA |