| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093853 |
| Chromosome: | chromosome 3 |
| Location: | 1222533 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149700 | (1 of 1) PF14518 - Iron-containing redox enzyme (Haem_oxygenas_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGATGGCCTGGTTGAGGCCAGCCGCCAGT |
| Internal bar code: | AGCGTACGTCACCTCCGGGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 80 |
| LEAP-Seq percent confirming: | 92.2175 |
| LEAP-Seq n confirming: | 3934 |
| LEAP-Seq n nonconfirming: | 332 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACGTTCCTCAAAACCCG |
| Suggested primer 2: | GAGGCGTAGAAGGTCGAGTG |