| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093871 |
| Chromosome: | chromosome 2 |
| Location: | 3957531 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096650 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAGTCGGCACGGGGGGCGGGGCCAGGC |
| Internal bar code: | GTCGTCCTCACGCCGCTGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 31 |
| LEAP-Seq percent confirming: | 0.339116 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 7641 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTGAACAAGCGAGCAGG |
| Suggested primer 2: | TAGCTGTAGCAGTGGCATGG |