Insertion junction: LMJ.RY0402.093871_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.093871
Chromosome:chromosome 8
Location:2563262
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g372500 (1 of 1) K11373 - elongator complex protein 1 (ELP1, IKI3, IKBKAP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACATCCCACACACACTCACACACGCATA
Internal bar code:GCCGCTTTGGGAGCTTTGATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:75
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTGGTCTGGTCTGGTCT
Suggested primer 2:CGATACCCACCATTTTACCG