| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093904 |
| Chromosome: | chromosome_2 |
| Location: | 2865198 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g094801 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGCTAGGTCACAAGTAAGCACCTCCCGCC |
| Internal bar code: | GGTTTGGTTTGGCGCCTTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 259 |
| LEAP-Seq percent confirming: | 77.194 |
| LEAP-Seq n confirming: | 3967 |
| LEAP-Seq n nonconfirming: | 1172 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCAGCTGTCGGTACCTT |
| Suggested primer 2: | TGTGATCGACTCCTCTGTGC |