Insertion junction: LMJ.RY0402.093930_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.093930
Chromosome:chromosome 10
Location:6247028
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g464800 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAAAATCGCACGTGCCATCCCCGCTGCTG
Internal bar code:CGCCCCCCGTGACTGGCTTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:454
LEAP-Seq percent confirming:97.2836
LEAP-Seq n confirming:3653
LEAP-Seq n nonconfirming:102
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGATGTTTGAGCTGGACA
Suggested primer 2:AACGTACTAACGTCCACGGC