| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.093956 |
| Chromosome: | chromosome 3 |
| Location: | 4190638 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173950 | (1 of 3) PF08495 - FIST N domain (FIST) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGATATTAATTTTCGGCGATATTTTTTT |
| Internal bar code: | TTTCCATGGCCGCGCTCTCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 18 |
| LEAP-Seq percent confirming: | 63.6384 |
| LEAP-Seq n confirming: | 2844 |
| LEAP-Seq n nonconfirming: | 1625 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGAGCACAGAGTGTTG |
| Suggested primer 2: | TGCAATTGGGTCGTGAATTA |