| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093957 |
| Chromosome: | chromosome 12 |
| Location: | 7228515 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558352 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGTGGCGGCGAAGACAAGACATAGGGGG |
| Internal bar code: | GCCTTCCGTTAATCGCGAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 877 |
| LEAP-Seq percent confirming: | 96.1552 |
| LEAP-Seq n confirming: | 8278 |
| LEAP-Seq n nonconfirming: | 331 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTTCCTCGCATTTGTTC |
| Suggested primer 2: | CGCATCACCAGAAGCAGTAA |