| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.093970 |
| Chromosome: | chromosome 5 |
| Location: | 370396 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233100 | KIN14-6,KIN14B,KIN14B-1 | (1 of 3) K10406 - kinesin family member C2/C3 (KIFC2_3); Kinesin motor protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAGCTGGAGGACCAGCAGCTCGAGCAG |
| Internal bar code: | TGCTCCTGACCTATGGTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 99.4071 |
| LEAP-Seq n confirming: | 1006 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATTCCCAGATCCTAAGCA |
| Suggested primer 2: | AGGTTCGTGTGTTTTGGGAG |