| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093974 |
| Chromosome: | chromosome 6 |
| Location: | 5097947 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280950 | PYK2 | Pyruvate kinase 2; (1 of 6) 2.7.1.40 - Pyruvate kinase / Phosphoenolpyruvate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTACCAGATTGCTCCCTGGTGCAGCAC |
| Internal bar code: | CCGGGAAAGCGCGAACGTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 48.9211 |
| LEAP-Seq n confirming: | 1859 |
| LEAP-Seq n nonconfirming: | 1941 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCGGGCTAAGCAAGATG |
| Suggested primer 2: | TTAGTTTCCCGGTCAAATGC |