| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094009 |
| Chromosome: | chromosome 11 |
| Location: | 2048223 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468600 | Hypothetical protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGCTGGCGCCCACGTCGCCGCTTTGAC |
| Internal bar code: | AGAGCTGCATAATCCTGAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 45.4618 |
| LEAP-Seq n confirming: | 1137 |
| LEAP-Seq n nonconfirming: | 1364 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGATGGCTGGCATGGTTT |
| Suggested primer 2: | ATTCCCACGTCGTAACTTGC |