| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094014 |
| Chromosome: | chromosome 10 |
| Location: | 6476774 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466300 | AAAS1 | (1 of 1) PTHR14494//PTHR14494:SF0 - ALADIN/ADRACALIN/AAAS // ALADIN; Aladin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGGGGTGCGGTGGTGGGCGTGAGGCAG |
| Internal bar code: | TGGGCCGGACACGTCGCGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 37 |
| LEAP-Seq percent confirming: | 5.26316 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTAGTCAGGGTCACGAGC |
| Suggested primer 2: | ACGACCTGACGAAAGTTGCT |