Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.094022 |
Chromosome: | chromosome 6 |
Location: | 5078869 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g280600 | GEX1 | Gamete-expressed protein 1; (1 of 1) PTHR33538:SF2 - PROTEIN GAMETE EXPRESSED 1 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAGCAGCACCTCAGGCGGCATCTGTCGC |
Internal bar code: | GTGGTACGCCCAGTCTGAATGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 629 |
LEAP-Seq percent confirming: | 98.9681 |
LEAP-Seq n confirming: | 4220 |
LEAP-Seq n nonconfirming: | 44 |
LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATGACCCCTGTGTGTGTGA |
Suggested primer 2: | GCGCATATAACGTAGGCCAT |