| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094078 |
| Chromosome: | chromosome 12 |
| Location: | 8239610 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550350 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCCCATGCTGCTACAGCCACAGGCGGA |
| Internal bar code: | CGCTCAAATTAGCACCTTCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 98.6487 |
| LEAP-Seq n confirming: | 292 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGTCTAGTTGCTGGTCC |
| Suggested primer 2: | AAGTGGGATATTGGCACTGG |