| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.094101 |
| Chromosome: | chromosome 12 |
| Location: | 8647247 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546800 | PHC53 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCTAACACCCACCTCATAGCTACCCGA |
| Internal bar code: | TAAAACCTGGGAGGAATTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 985 |
| LEAP-Seq percent confirming: | 99.7417 |
| LEAP-Seq n confirming: | 5019 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGGTCGGTCGGTAACTG |
| Suggested primer 2: | AACCACGTAGTGAAGCCCAC |