Insertion junction: LMJ.RY0402.094104_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.094104
Chromosome:chromosome 3
Location:4390529
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g175500 (1 of 2) K05770 - benzodiazapine receptor (BZRP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCATTGGAGATATGGAGATCGACAAGAAG
Internal bar code:CAGGAATCTAGGAAAAGATAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:694
LEAP-Seq percent confirming:98.434
LEAP-Seq n confirming:5217
LEAP-Seq n nonconfirming:83
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:AAAACCACTATCCACTGGCG
Suggested primer 2:CTTCCCATCGTTTGTCAGGT