| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094110 |
| Chromosome: | chromosome 12 |
| Location: | 3271520 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g498150 | (1 of 2) PTHR22847//PTHR22847:SF431 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGGGGCCAGCCACTGCACATGAGGA |
| Internal bar code: | CAGATTGGGGCTAACGACCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1396 |
| LEAP-Seq percent confirming: | 99.0675 |
| LEAP-Seq n confirming: | 1806 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGATGAAGTTGGCAAGCAG |
| Suggested primer 2: | GTGCAGGTGAGGAAGAGCA |