Insertion junction: LMJ.RY0402.094124_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.094124
Chromosome:chromosome 1
Location:3446618
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g021950 (1 of 1) PF08393//PF12777//PF12781 - Dynein heavy chain, N-terminal region 2 (DHC_N2) // Microtubule-binding stalk of dynein motor (MT) // ATP-binding dynein motor region D5 (AAA_9) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCTACTCCGCGGCGCGGGTGATGGAGCT
Internal bar code:TTGAATCTCATCGCTGGGGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:69
LEAP-Seq percent confirming:52.5424
LEAP-Seq n confirming:93
LEAP-Seq n nonconfirming:84
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCAACATCCAACAACAC
Suggested primer 2:GGCAGTTCCAGCGTGTAGTC