| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094134 |
| Chromosome: | chromosome 6 |
| Location: | 2994763 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273450 | POB11 | Proteome of basal body 11 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCTTTCACACCGCTTCAATGCCCACTT |
| Internal bar code: | GCGCATGGAACAAGGGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 40.6504 |
| LEAP-Seq n confirming: | 450 |
| LEAP-Seq n nonconfirming: | 657 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGACTTTGGGAAACTGG |
| Suggested primer 2: | CTTTACGATGCTGCCTGACA |