| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094138 |
| Chromosome: | chromosome 1 |
| Location: | 5596406 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039702 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTGCCTGAAACTTGAAACAACTCGTCA |
| Internal bar code: | CCGTCCCTAGTTGGAATTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 88.8176 |
| LEAP-Seq n confirming: | 3320 |
| LEAP-Seq n nonconfirming: | 418 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCGGTCAAACGTCAACC |
| Suggested primer 2: | AAGGTGCTGCCTCCTGTAGA |