| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094180 |
| Chromosome: | chromosome 6 |
| Location: | 5277303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282700 | (1 of 1) PTHR24414//PTHR24414:SF29 - FAMILY NOT NAMED // SEGMENTATION PROTEIN CAP'N'COLLAR | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTATAATAAAGCAGGATGAGGACGTGCA |
| Internal bar code: | AGGGACACCGTTTATCCCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.252 |
| LEAP-Seq n confirming: | 1725 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGAAGGCAAAAGGGAATC |
| Suggested primer 2: | GAGGACAGCGAGTTGGAGAG |