| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094183 |
| Chromosome: | chromosome 10 |
| Location: | 859086 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423800 | KU80 | (1 of 1) K10885 - ATP-dependent DNA helicase 2 subunit 2 (XRCC5, KU80, G22P2); ATP-dependent DNA helicase 2 subunit KU80 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGACGAAGAAGCGGCATGGACTTGAAAGG |
| Internal bar code: | CAGGTGAAGAAGCGGGGGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 306 |
| LEAP-Seq percent confirming: | 71.4948 |
| LEAP-Seq n confirming: | 617 |
| LEAP-Seq n nonconfirming: | 246 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTTCGTATGGCACTCA |
| Suggested primer 2: | TCTCACATGCTCACTCCCTG |