| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094187 |
| Chromosome: | chromosome 16 |
| Location: | 7366784 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685053 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGAGGCGGATCTCATGCCACAAAATCG |
| Internal bar code: | CATCGGAGTTGTCGACTCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 97.4082 |
| LEAP-Seq n confirming: | 5863 |
| LEAP-Seq n nonconfirming: | 156 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTTCGTCAGCTCGTCTC |
| Suggested primer 2: | GTGCAGAATGTGGTGACAGG |