Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.094213 |
Chromosome: | chromosome 17 |
Location: | 478120 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g699200 | (1 of 1) IPR000104//IPR000626//IPR029071 - Antifreeze protein, type I // Ubiquitin domain // Ubiquitin-related domain | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCAGGTGGCGGGGGCAGCAGCGCCCCGC |
Internal bar code: | GTCCTGCCCTTATCAGTTGGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 225 |
LEAP-Seq percent confirming: | 82.0539 |
LEAP-Seq n confirming: | 1614 |
LEAP-Seq n nonconfirming: | 353 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTACATGCAAGGTCCCAGGT |
Suggested primer 2: | AGAGAAGGCAAGAGCACGAC |