| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094214 |
| Chromosome: | chromosome 6 |
| Location: | 3913968 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278183 | (1 of 1) PTHR23054//PTHR23054:SF14 - UNCHARACTERIZED // PROTEIN Y45F10A.7, ISOFORM A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGCCGCCCACGAAGGCCTCCGCCGCCG |
| Internal bar code: | GTGTGGATTATGGACAGAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 88.2283 |
| LEAP-Seq n confirming: | 2968 |
| LEAP-Seq n nonconfirming: | 396 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTGGCTAGGACTCACTG |
| Suggested primer 2: | GACATCGGTGAGACTGGGTT |