Insertion junction: LMJ.RY0402.094226_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.094226
Chromosome:chromosome 3
Location:4331588
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g174950 TGL7 (1 of 3) PTHR21493//PTHR21493:SF87 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // SN1-SPECIFIC DIACYLGLYCEROL LIPASE BETA; Putative triacylglycerol lipase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTGGCAGCCCAGACGGGCCGTGTGATGC
Internal bar code:GAGGGGTTGAAGTAGGACGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:81
LEAP-Seq percent confirming:44.4375
LEAP-Seq n confirming:707
LEAP-Seq n nonconfirming:884
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCATCACTCCATGTCCAATG
Suggested primer 2:GCATATTGAGGAGCTAGCCG