Insertion junction: LMJ.RY0402.094236_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.094236
Chromosome:chromosome 2
Location:4057166
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g097250 transportin-3 family protein; (1 of 1) K15436 - transportin-3 (TRPO3, MTR10) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAACGCCCCTGCTCCTCCTCCACTCCCGC
Internal bar code:TTTGTACATTTCTTTGGTTCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:153
LEAP-Seq percent confirming:99.3355
LEAP-Seq n confirming:299
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGGACAGTGCCAAGGGTATG
Suggested primer 2:CTCCAGCTGTTGTAGCCCTC