| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094251 |
| Chromosome: | chromosome 12 |
| Location: | 6968286 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560200 | NAT2 | Glucosamine 6-phosphate N-acetyltransferase; (1 of 1) 2.3.1.4 - Glucosamine-phosphate N-acetyltransferase / Phosphoglucosamine transacetylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCCACGCTGCAAATCTTGCCTCGACAG |
| Internal bar code: | CCTTCCGTTTGGTACTCTCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 73.7983 |
| LEAP-Seq n confirming: | 3915 |
| LEAP-Seq n nonconfirming: | 1390 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACTTGCAGGTTGTGATT |
| Suggested primer 2: | GCTGAACCGAATGGTAAGGA |