| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094262 |
| Chromosome: | chromosome 1 |
| Location: | 5030956 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034800 | (1 of 1) PTHR21477//PTHR21477:SF12 - FAMILY NOT NAMED // CF9 PROTEIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACGGCTCTCTGGCAAGCACCCACTCCC |
| Internal bar code: | TTTGGTGGTGGACCGGTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6 |
| LEAP-Seq percent confirming: | 92.5373 |
| LEAP-Seq n confirming: | 124 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACTTCCGAGCAGTCAAA |
| Suggested primer 2: | CCGGCTACAACCTGTACGAT |