| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094264 |
| Chromosome: | chromosome 7 |
| Location: | 5523211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351100 | THB5,PRX | (1 of 1) IPR000104//IPR001486//IPR009050 - Antifreeze protein, type I // Truncated hemoglobin // Globin-like; Truncated hemoglobin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCAGGCGCAATTAACAACCGATGATT |
| Internal bar code: | TGAAGCGGGTGTTTAAGTCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 99.6961 |
| LEAP-Seq n confirming: | 26572 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGTGACTTCATGGCAGC |
| Suggested primer 2: | TCGTTGTGTGTGAGGTGGAT |