Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.094285 |
Chromosome: | chromosome 2 |
Location: | 5255592 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g106300 | (1 of 1) PTHR12883:SF0 - COILED-COIL DOMAIN-CONTAINING PROTEIN 47 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGATATGGCGCCCTACGGCGCTGCA |
Internal bar code: | CTGGCGCCTGTAAATAAGCGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 715 |
LEAP-Seq percent confirming: | 98.7677 |
LEAP-Seq n confirming: | 13064 |
LEAP-Seq n nonconfirming: | 163 |
LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATATGTTCCTTTGTTGCGGC |
Suggested primer 2: | TTTGCCCTTCATTATCCTGC |