| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094324 |
| Chromosome: | chromosome 9 |
| Location: | 1283303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399200 | SDR19 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTGACATGCATCAAGCACAAACGCGTG |
| Internal bar code: | GGAAGCCCCCTCACTGTACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 90.5427 |
| LEAP-Seq n confirming: | 6290 |
| LEAP-Seq n nonconfirming: | 657 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCTATCGCCACAACAGGT |
| Suggested primer 2: | TTGAGCGTAGCGTCAAAATG |