| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.094340 |
| Chromosome: | chromosome 11 |
| Location: | 2382438 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g474700 | BES6 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin); putative chloride channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGCATGGGTGACGTTGTTCACAGCAGG |
| Internal bar code: | AATCCGAGCGCGACAGAGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 3.12587 |
| LEAP-Seq n confirming: | 112 |
| LEAP-Seq n nonconfirming: | 3471 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACGATGGCTAAACCCTG |
| Suggested primer 2: | CGTATGGTGAGAACGTGGTG |