Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.094345 |
Chromosome: | chromosome 2 |
Location: | 6356930 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g115350 | (1 of 2) 2.7.7.59 - [Protein-PII] uridylyltransferase / Uridylyl removing enzyme | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTGCAAAAGCTGCCCCAGCGGCATATG |
Internal bar code: | CGTACAGACGTTCGGTCATCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1 |
LEAP-Seq percent confirming: | 84.3562 |
LEAP-Seq n confirming: | 2378 |
LEAP-Seq n nonconfirming: | 441 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGTGTGTGATGGGACAGGGT |
Suggested primer 2: | TCCCCAAATGAAGACCTACG |