| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094345 |
| Chromosome: | chromosome 14 |
| Location: | 1834487 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g620250 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGTGACCTGCGTGTGCAGCAAGCAATTG |
| Internal bar code: | AAATTGCGGTACCAGCCTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 88.7287 |
| LEAP-Seq n confirming: | 3385 |
| LEAP-Seq n nonconfirming: | 430 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTTATCCGCGTTAGCTGC |
| Suggested primer 2: | ATTCAATGGATGGAGCAAGC |