| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094359 |
| Chromosome: | chromosome 6 |
| Location: | 7244729 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298225 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCGCCGGTGCCAGAGCTGTTTAGGCA |
| Internal bar code: | CGGCCTTGGCTCACAGGCACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 98.5618 |
| LEAP-Seq n confirming: | 18024 |
| LEAP-Seq n nonconfirming: | 263 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTATGTGTCAGCGGTGG |
| Suggested primer 2: | AGGACTTTGGGGTGAGTGTG |