Insertion junction: LMJ.RY0402.094362_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.094362
Chromosome:chromosome 2
Location:5258274
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g106350 CGL68,HAP5 (1 of 1) PTHR31446:SF5 - ACID PHOSPHATASE/VANADIUM-DEPENDENT HALOPEROXIDASE-RELATED PROTEIN; Acid phosphatase/vanadium-dependent haloperoxidase related, DUF212 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTATGGGAGGGAGGTTAAGGGGCAGCGGG
Internal bar code:ACTAGTATGGCCGCGCTTAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:275
LEAP-Seq percent confirming:99.6198
LEAP-Seq n confirming:524
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCCCAAGTCCAGTCCCT
Suggested primer 2:CCGTGCTCAACATCATCATC