| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.094382 |
| Chromosome: | chromosome 9 |
| Location: | 7607003 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414750 | ELG33 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 33 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGCTTGGTTTGGTTTAGTTTGGGCTA |
| Internal bar code: | ACATGTTTACGTGCGAGGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 97.4843 |
| LEAP-Seq n confirming: | 1240 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTGACGTTGCAGTATCC |
| Suggested primer 2: | TGCGAATATTTGACGGTTGA |