| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094385 |
| Chromosome: | chromosome 11 |
| Location: | 1128963 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467680 | (1 of 13) 3.1.13.4 - Poly(A)-specific ribonuclease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCTGCAGCTGTTGCGGCTGTTGCGAG |
| Internal bar code: | GCGGTGTTTCGACGGCGAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 214 |
| LEAP-Seq percent confirming: | 99.2634 |
| LEAP-Seq n confirming: | 539 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGGATCTCTTTTATGCAA |
| Suggested primer 2: | GTCATTGTCGTCGTCAGCAG |