| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094385 |
| Chromosome: | chromosome 15 |
| Location: | 369988 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAAGGGTGATGCCAACCAGCACGATCC |
| Internal bar code: | GACAAAGGCAGGGGTTTGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 1.92308 |
| LEAP-Seq n confirming: | 153 |
| LEAP-Seq n nonconfirming: | 7803 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCACACTCTCCGCTGAA |
| Suggested primer 2: | CAGTGCACGTACCCAACATC |