Insertion junction: LMJ.RY0402.094391_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.094391
Chromosome:chromosome 3
Location:5792495
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g188850 (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGCAATCACCACATTCAACGCGTCACCA
Internal bar code:CGTGGTGTAATGCCGTAAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:161
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:169
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGTACGGCTGCTAACCACAG
Suggested primer 2:ACCCACTCCTAACGTCATGC