| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094417 |
| Chromosome: | chromosome 17 |
| Location: | 4542211 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732650 | (1 of 1) K17601 - WD repeat-containing protein 81 (WDR81) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCCGACATACAGCCGCCGCAGCTTTCCA |
| Internal bar code: | GCAAAGGAGGCGCTTCTATTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 98.5816 |
| LEAP-Seq n confirming: | 417 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCCTTGAGCTTGGCTTC |
| Suggested primer 2: | CACTTGGTGTTATTCCGCCT |