Insertion junction: LMJ.RY0402.094431_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.094431
Chromosome:chromosome 16
Location:435216
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g693203 (1 of 5) 3.6.3.27 - Phosphate-transporting ATPase / ABC phosphate transporter intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACACACCACCAACGCACCTCCCACCACAC
Internal bar code:GGGCAAGTTTTCCCCCCGCCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:412
LEAP-Seq percent confirming:61.8375
LEAP-Seq n confirming:875
LEAP-Seq n nonconfirming:540
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCCACCCTCTCACACAC
Suggested primer 2:AAGGAGCTCTCGTACTTGCG