| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.094431 |
| Chromosome: | chromosome 16 |
| Location: | 435227 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693203 | (1 of 5) 3.6.3.27 - Phosphate-transporting ATPase / ABC phosphate transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGATGAAGGATTGTTTGTGGGGACTGG |
| Internal bar code: | TGGAACTAGGTAGCAGTTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 93.1183 |
| LEAP-Seq n confirming: | 1299 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCCACCCTCTCACACAC |
| Suggested primer 2: | AAGGAGCTCTCGTACTTGCG |