| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094465 |
| Chromosome: | chromosome 9 |
| Location: | 6558460 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408000 | POB22 | (1 of 3) PF03227 - Gamma interferon inducible lysosomal thiol reductase (GILT) (GILT); Proteome of basal body 22 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGCCCCTCATCTTCATATGCTTGCACG |
| Internal bar code: | AGGTTCACACGGAGGGAGCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 99.0409 |
| LEAP-Seq n confirming: | 5060 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAATTCCAATGGAGGAGAA |
| Suggested primer 2: | AGATGGTGATGATGGTGGGT |