| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.094467 |
| Chromosome: | chromosome 6 |
| Location: | 6205234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g290550 | (1 of 1) PTHR23213//PTHR23213:SF243 - FORMIN-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGACAGGCAGTCACTCCGCCCTACCTC |
| Internal bar code: | AGTCCGCTTAGACTACTGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 99.5846 |
| LEAP-Seq n confirming: | 959 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCTTGGTGGACAGGTTT |
| Suggested primer 2: | GTCCATGTACACGTCGATGC |