| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094469 |
| Chromosome: | chromosome 3 |
| Location: | 4984478 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180100 | UNG2 | Uracil DNA glycosylase; (1 of 1) 3.2.2.29 - Thymine-DNA glycosylase / Uracil/thymine DNA glycosylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTGCAGGCGCGGGAGGGGAAGCGGCAGC |
| Internal bar code: | TGGTGGTACGTGCAGTGCAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 98.7375 |
| LEAP-Seq n confirming: | 2268 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCTGAGCTGGGGTCTTGA |
| Suggested primer 2: | GGTTGCTTAACAACGACGGT |