| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094472 |
| Chromosome: | chromosome 12 |
| Location: | 8270318 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGTAGCGGTGGCAGTAGCAACAATGGT |
| Internal bar code: | CCAATGCGTCAATTCGGAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 26.2781 |
| LEAP-Seq n confirming: | 257 |
| LEAP-Seq n nonconfirming: | 721 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTTGCTAAGACAGCCG |
| Suggested primer 2: | GTTCCAGGACTTCCAACCAA |