| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094479 |
| Chromosome: | chromosome 4 |
| Location: | 4057932 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g232902 | (1 of 77) IPR012340 - Nucleic acid-binding, OB-fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTTGCCTTCAGCCCAAGGCGTCCATACT |
| Internal bar code: | CGGGTGGCTAGGGCAAATCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 97.9266 |
| LEAP-Seq n confirming: | 4534 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGAGAACAATTGCAGG |
| Suggested primer 2: | TTTTCAGCTGGTGTGAGTCG |