| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094480 |
| Chromosome: | chromosome 3 |
| Location: | 6167713 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192201 | PHC51 | Pherophorin-chlamydomonas homolog 51; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGGCTCCTGCTGCCGGGAATGGCCCAC |
| Internal bar code: | GAGCGGAGAACACCCCGTTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 610 |
| LEAP-Seq percent confirming: | 99.5072 |
| LEAP-Seq n confirming: | 2221 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTGCGAAAACGACTTGA |
| Suggested primer 2: | CTTTCCCTTCCATCCTCTCC |